| Gene | Ccdc83 |
| Chromosome | 7 |
| Deletion Start | 90,259,521 |
| Deletion End | 90,236,196 |
| Deletion Size | 23,326 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,258,802 | 90,258,825 | GACAAGTGCTGAGCCAGAATACAG |
| TUP | 90,258,778 | 90,258,800 | TGTGAGCAGGACCACATGGTTCC |
| TUR | 90,258,699 | 90,258,720 | TCCAAACCCTCTGACTAGAGAC |
| TDF | 90,236,583 | 90,236,604 | GTGTCCTCAAACCAAGCTAGTG |
| TDP | 90,236,557 | 90,236,578 | TCATGAGCCTGCCTCCTGCTGG |
| TDR | 90,236,467 | 90,236,489 | GAAGGCTGAGCACATTTCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | AGTACTCATGCCTTACACAG |
| SD | ACTGGGAAAGGAGTTCTCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 127 bp | + | + | - |
| TDF | TDR | 138 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15459A-G3 | VGB6 | C57BL/6NTac | |
| 15459B-A6 | VGB6 | C57BL/6NTac | |
