| Gene | Art5 |
| Chromosome | 7 |
| Deletion Start | 102,099,490 |
| Deletion End | 102,097,020 |
| Deletion Size | 2,471 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,098,766 | 102,098,790 | GCAATTATCCTCATGCTCTCCTCAC |
| TUP | 102,098,742 | 102,098,763 | CCCTGTGAGGCTGGAAGCATCC |
| TUR | 102,098,698 | 102,098,719 | CTGGCTCTGGGATCTGGACTTC |
| TDF | 102,097,273 | 102,097,294 | CAGCCTGCTCTGAGACTTGTTC |
| TDP | 102,097,250 | 102,097,271 | AGCTTCACTGGCCCTGCTGCCC |
| TDR | 102,097,212 | 102,097,230 | GCCCGACTGCTGGAGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CGCAAAGAAGTCCTGGAGAC |
| SD | GAAGCAGGAGACCTGAGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15460A-A7 | VGB6 | C57BL/6NTac | |
| 15460A-H7 | VGB6 | C57BL/6NTac | |
| 15460A-E8 | VGB6 | C57BL/6NTac | |
| 15460A-G7 | VGB6 | C57BL/6NTac | |
| 15460A-E3 | VGB6 | C57BL/6NTac | |
