Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

15462 Hpx Status: ES cell colonies screened / QC positives

Gene	Hpx
Chromosome	7
Deletion Start	105,600,087
Deletion End	105,591,681
Deletion Size	8,407
Genome Build	38

MGI ID	MGI:105112
NCBI Gene ID	15458
BAC ID	125M9
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	105,600,052	105,600,073	GGCGCTAAATATCCTGGTGTTG
TUP	105,600,032	105,600,051	CTGGGCCTGTGCTGGTCCCT
TUR	105,599,982	105,600,005	CTATCCCTAATTCTGGCCCTACTC
TDF	105,592,227	105,592,247	CCCAGGGCACTCAAGTGTATG
TDP	105,592,200	105,592,226	TCTTCCTGACAAAGGGAGGCAATAACC
TDR	105,592,154	105,592,173	GGGCTCCCAAGTTCCTTCTC

PCR Primers

Primer	Sequence
SU	TGAGTTGGACCTGACATTGG
SD	AGAGTTGATAACTTTTCAATCC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	92 bp	+	+	-
TDF	TDR	94 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
15462A-B6	VGB6	C57BL/6NTac
15462A-D3	VGB6	C57BL/6NTac
15462A-G7	VGB6	C57BL/6NTac
15462A-H12	VGB6	C57BL/6NTac