Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

15465 Tmem9b Status: ES cell colonies screened / QC positives

Gene	Tmem9b
Chromosome	7
Deletion Start	109,752,175
Deletion End	109,736,789
Deletion Size	15,387
Genome Build	38

MGI ID	MGI:1915254
NCBI Gene ID	56786
BAC ID	327P13
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	109,751,713	109,751,736	CCCTCATCTCCCTGAAATCTTCTC
TUP	109,751,688	109,751,709	TAGGGACTAGCTCCCCTCACCC
TUR	109,751,618	109,751,641	GGCAAGGGAATCTGCTTATCAATG
TDF	109,736,867	109,736,884	CCAGGAGCAGCGAAAGTC
TDP	109,736,844	109,736,866	TGTCTTCGACCGACACGTTGTCC
TDR	109,736,790	109,736,810	CCAGTTGTCTGCGTGTTCTTC

PCR Primers

Primer	Sequence
SU	TCTTCTGGTCAGGTCTCCTG
SD	TGGCAAGAAAGAGAGAGCTTG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	119 bp	+	+	-
TDF	TDR	95 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
15465A-A7	VGB6	C57BL/6NTac
15465A-C11	VGB6	C57BL/6NTac
15465A-E2	VGB6	C57BL/6NTac
15465A-G11	VGB6	C57BL/6NTac