| Gene | Scnn1g |
| Chromosome | 7 |
| Deletion Start | 121,767,651 |
| Deletion End | 121,737,921 |
| Deletion Size | 29,731 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,738,520 | 121,738,541 | CTTCCCATGTTCACTCCCTTAC |
| TUP | 121,738,542 | 121,738,563 | TCTCACCCAAAGCCAGGCTGAC |
| TUR | 121,738,565 | 121,738,584 | TCACCTGGGTGTCACTTCTG |
| TDF | 121,767,165 | 121,767,185 | GCAGATTGAGATGCTCCTGTC |
| TDP | 121,767,187 | 121,767,209 | AACTTTGGTGGACAGCTCGGCCT |
| TDR | 121,767,292 | 121,767,311 | TCCTTGGCTTTCTGCCACTG |
PCR Primers
| Primer | Sequence |
| SU | AGAACTCCAGGGAAAGGTCC |
| SD | GAGGACACCTTGTCCCAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 147 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15466A-A9 | VGB6 | C57BL/6NTac | |
| 15466A-C4 | VGB6 | C57BL/6NTac | |
| 15466A-H12 | VGB6 | C57BL/6NTac | |
| 15466A-H2 | VGB6 | C57BL/6NTac | |
| 15466A-F12 | VGB6 | C57BL/6NTac | |
