15466   Scnn1g     Status: ES cell colonies screened / QC positives

GeneScnn1g
Chromosome7
Deletion Start121,767,651
Deletion End121,737,921
Deletion Size29,731
Genome Build38
MGI IDMGI:104695
NCBI Gene ID20278
BAC ID170P17
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF121,738,520121,738,541CTTCCCATGTTCACTCCCTTAC
TUP121,738,542121,738,563TCTCACCCAAAGCCAGGCTGAC
TUR121,738,565121,738,584TCACCTGGGTGTCACTTCTG
TDF121,767,165121,767,185GCAGATTGAGATGCTCCTGTC
TDP121,767,187121,767,209AACTTTGGTGGACAGCTCGGCCT
TDR121,767,292121,767,311TCCTTGGCTTTCTGCCACTG

PCR Primers

PrimerSequence
SUAGAACTCCAGGGAAAGGTCC
SDGAGGACACCTTGTCCCAGTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR65 bp++-
TDFTDR147 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15466A-A9VGB6C57BL/6NTac
15466A-C4VGB6C57BL/6NTac
15466A-H12VGB6C57BL/6NTac
15466A-H2VGB6C57BL/6NTac
15466A-F12VGB6C57BL/6NTac