| Gene | Phkg2 |
| Chromosome | 7 |
| Deletion Start | 127,583,124 |
| Deletion End | 127,573,875 |
| Deletion Size | 9,250 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,574,378 | 127,574,397 | GGGAGCAGGGATTGCTACTG |
| TUP | 127,574,398 | 127,574,424 | TGATCGCCACCACAGATGCTTTATGGT |
| TUR | 127,574,493 | 127,574,513 | TTCCTCAGGGTTCCTGTTCTG |
| TDF | 127,582,955 | 127,582,973 | CCCATTGCTGCCACTGAAC |
| TDP | 127,582,975 | 127,582,997 | CGAAGGAGACTCCGGTGCCATCA |
| TDR | 127,583,034 | 127,583,051 | TGGCTTGCCCAGGGATAC |
PCR Primers
| Primer | Sequence |
| SU | GTCATCCTGCTCGAGACCC |
| SD | AGCAAAGGCAAGACCAAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 136 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15467A-A11 | VGB6 | C57BL/6NTac | |
| 15467A-D10 | VGB6 | C57BL/6NTac | |
| 15467A-E1 | VGB6 | C57BL/6NTac | |
| 15467A-F4 | VGB6 | C57BL/6NTac | |
| 15467A-G12 | VGB6 | C57BL/6NTac | |
| 15467A-H5 | VGB6 | C57BL/6NTac | |
