| Gene | Hmx2 |
| Chromosome | 7 |
| Deletion Start | 131,556,018 |
| Deletion End | 131,554,310 |
| Deletion Size | 1,709 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,554,836 | 131,554,853 | TGCGGGCGGTACACAATG |
| TUP | 131,554,861 | 131,554,887 | AAATGGTTTCGCACGCCGGGTACTTTC |
| TUR | 131,554,909 | 131,554,930 | TGACAGAACACCCAAACCAGAC |
| TDF | 131,544,390 | 131,544,409 | CAGAATCGCCGCAACAAGTG |
| TDP | 131,555,771 | 131,555,790 | AAGCGGCAGCTCTCAGCCGA |
| TDR | 131,555,851 | 131,555,871 | AGCAGCGAACTGTCCCGAAAC |
PCR Primers
| Primer | Sequence |
| SU | ATGTTACGGGCTGGACAGAG |
| SD | GGCTGAGCTTGTGTCCTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 11482 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15471A-E6 | VGB6 | C57BL/6NTac | |
| 15471A-A10 | VGB6 | C57BL/6NTac | |
| 15471A-B3 | VGB6 | C57BL/6NTac | |
| 15471A-C11 | VGB6 | C57BL/6NTac | |
| 15471A-A9 | VGB6 | C57BL/6NTac | |
| 15471A-C7 | VGB6 | C57BL/6NTac | |
