| Gene | 1700018L24Rik |
| Chromosome | 8 |
| Deletion Start | 11,855,707 |
| Deletion End | 11,840,922 |
| Deletion Size | 14,786 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,841,706 | 11,841,726 | GGGAGGCATTAGGTCATGCTG |
| TUP | 11,841,730 | 11,841,755 | CCAGACACCTCCACAGGCCAAAGACT |
| TUR | 11,841,785 | 11,841,804 | GGCATGGCTTCCTTGTTCTG |
| TDF | 11,855,216 | 11,855,237 | CCAGAAGAAGGCTGGGTTAGAG |
| TDP | 11,855,245 | 11,855,271 | TGTCCCAAAGGTGGCATCTAGTTGTGG |
| TDR | 11,855,296 | 11,855,315 | AGGGCATGAGGGTGAGTGTC |
PCR Primers
| Primer | Sequence |
| SU | AAGCTGAGGTAGCCAGTTCG |
| SD | GTTAGGACAGTGTTCTTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15477A-E6 | VGB6 | C57BL/6NTac | |
| 15477A-E12 | VGB6 | C57BL/6NTac | |
| 15477A-F7 | VGB6 | C57BL/6NTac | |
| 15477A-G9 | VGB6 | C57BL/6NTac | |
