| Gene | Ces1e |
| Chromosome | 8 |
| Deletion Start | 93,229,418 |
| Deletion End | 93,201,317 |
| Deletion Size | 28,102 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,228,919 | 93,228,939 | CCTCTCCACTGGGTCACTTTG |
| TUP | 93,228,896 | 93,228,918 | AGGATGCCTTAGGTGGCGCCTTC |
| TUR | 93,228,847 | 93,228,868 | CCCTCTTATCCGTCATCTCCTC |
| TDF | 93,201,842 | 93,201,862 | GGGCCAACTTTGCTAGGAATG |
| TDP | 93,201,814 | 93,201,839 | TGAGTCTAGTGATGCAGATGGAGCCA |
| TDR | 93,201,792 | 93,201,812 | TGTCTCATCTCACCCATCAAC |
PCR Primers
| Primer | Sequence |
| SU | ACCAGTTTGTGGACTCCTGC |
| SD | TGTGTGTGACTCCTCCTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15493A-G2 | VGB6 | C57BL/6NTac | |
| 15493B-A2 | VGB6 | C57BL/6NTac | |
| 15493B-A9 | VGB6 | C57BL/6NTac | |
| 15493B-B12 | VGB6 | C57BL/6NTac | |
| 15493B-E11 | VGB6 | C57BL/6NTac | |
