| Gene | Ces4a |
| Chromosome | 8 |
| Deletion Start | 105,149,585 |
| Deletion End | 105,131,976 |
| Deletion Size | 17,610 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,132,192 | 105,132,211 | TGCAAAGTGAGGACTGTGTC |
| TUP | 105,132,212 | 105,132,235 | TCCTACCTCCTGGGATCAGCGAGG |
| TUR | 105,132,236 | 105,132,257 | GGGCAAGTCATGTTCTCAGGTC |
| TDF | 105,149,140 | 105,149,162 | TGGCCTCAAACTCAACAATTCTC |
| TDP | 105,149,167 | 105,149,191 | CTTCACCTCTGGAGTGCTGGGACTC |
| TDR | 105,149,201 | 105,149,222 | GAGCTAACTGGGTGTGATGGTG |
PCR Primers
| Primer | Sequence |
| SU | GTCTGAGGGCTCCAGTCTTG |
| SD | TGGCAGTGTGTGAACTTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15497A-A12 | VGB6 | C57BL/6NTac | |
| 15497A-C2 | VGB6 | C57BL/6NTac | |
| 15497A-C4 | VGB6 | C57BL/6NTac | |
| 15497A-D4 | VGB6 | C57BL/6NTac | |
| 15497A-H10 | VGB6 | C57BL/6NTac | |
| 15497A-F5 | VGB6 | C57BL/6NTac | |
