| Gene | Elmo3 |
| Chromosome | 8 |
| Deletion Start | 105,309,906 |
| Deletion End | 105,305,755 |
| Deletion Size | 4,152 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,306,561 | 105,306,583 | AACCACATCCATCTTTCCTCTAC |
| TUP | 105,306,584 | 105,306,606 | TGCTCCTGGCAGGATCTGAAGGC |
| TUR | 105,306,636 | 105,306,654 | CAGCACCCTTCACGACTTG |
| TDF | 105,308,961 | 105,308,979 | AATCCGCCAGCAGCGTTTG |
| TDP | 105,308,981 | 105,309,007 | TCCGACTCTGTGAAGGAATGCTCTTCC |
| TDR | 105,309,039 | 105,309,059 | CCACACCATCAGCCCATTCAG |
PCR Primers
| Primer | Sequence |
| SU | CTGGGTGGGTCACCTTACTG |
| SD | ACATTCCTGTCGCTTCTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15498A-A6 | VGB6 | C57BL/6NTac | |
| 15498A-B4 | VGB6 | C57BL/6NTac | |
| 15498A-D9 | VGB6 | C57BL/6NTac | |
| 15498A-G2 | VGB6 | C57BL/6NTac | |
| 15498A-F7 | VGB6 | C57BL/6NTac | |
| 15498A-B12 | VGB6 | C57BL/6NTac | |
