| Gene | Calb2 |
| Chromosome | 8 |
| Deletion Start | 110,168,134 |
| Deletion End | 110,156,924 |
| Deletion Size | 11,211 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,168,053 | 110,168,075 | CCTGGAAATCTGGAAGCACTTTG |
| TUP | 110,168,028 | 110,168,051 | TGCTGACGGTCAGTAAAGCTCCCA |
| TUR | 110,167,947 | 110,167,969 | GAAGCATACCTACCCGCATTGAG |
| TDF | 110,157,411 | 110,157,433 | GTCTCACTCTAAGGCTCCCAATG |
| TDP | 110,157,385 | 110,157,408 | TGCTGGGAGGACACTCTCCAACTC |
| TDR | 110,157,339 | 110,157,358 | TGCGACTAGGGCAGAAGTTG |
PCR Primers
| Primer | Sequence |
| SU | TGTGCTCAAGATGGACAGAG |
| SD | ATCCATGCACACATAAGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 129 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15501A-G4 | VGB6 | C57BL/6NTac | |
| 15501A-C6 | VGB6 | C57BL/6NTac | |
| 15501A-F9 | VGB6 | C57BL/6NTac | |
| 15501A-E11 | VGB6 | C57BL/6NTac | |
| 15501A-E5 | VGB6 | C57BL/6NTac | |
| 15501A-D12 | VGB6 | C57BL/6NTac | |
