15502   Ftsjd1     Status: ES cell colonies screened / QC positives

GeneFtsjd1
Chromosome8
Deletion Start110,223,373
Deletion End110,221,063
Deletion Size2,311
Genome Build38
MGI IDMGI:2384580
NCBI Gene ID234728
BAC ID407I7
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 8

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF110,221,731110,221,752CGGCATGTCTCCCATTCACTTG
TUP110,221,754110,221,776TCACGGCTGATGGCAGCTTCGAT
TUR110,221,784110,221,805GCTTCCTGTTCACCTGGGTTTC
TDF110,222,914110,222,934CCAGCCTACCAGCATTTGTTC
TDP110,222,935110,222,956TTGGACTGCCTGCTGCACTCAC
TDR110,222,986110,223,006GCAAGAAAGGATGGGCAAGAC

PCR Primers

PrimerSequence
SUAGCCAGGTGAGTTGCTATGG
SDGTCTGAATGAAGGCACAGGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR75 bp++-
TDFTDR93 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15502A-A12VGB6C57BL/6NTac
15502A-C7VGB6C57BL/6NTac
15502A-C9VGB6C57BL/6NTac
15502A-G6VGB6C57BL/6NTac
15502A-C12VGB6C57BL/6NTac
15502A-F11VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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