| Gene | Gcsh |
| Chromosome | 8 |
| Deletion Start | 116,993,423 |
| Deletion End | 116,982,602 |
| Deletion Size | 10,822 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,992,714 | 116,992,732 | CTGGGAGACCTGCCCATTG |
| TUP | 116,992,689 | 116,992,711 | CGCGTTTGCACAGTTAGCTGGGA |
| TUR | 116,992,639 | 116,992,660 | CGATTTGTGCTGCCTGAGTAAG |
| TDF | 116,982,739 | 116,982,760 | GGCAATGTGAACTGAGGTTCTG |
| TDP | 116,982,709 | 116,982,736 | TTGGGTACAGGTTGGCTGATCAAGATGA |
| TDR | 116,982,624 | 116,982,647 | CATGGTCACTCCTCAATGGACTTC |
PCR Primers
| Primer | Sequence |
| SU | CTAGATTGCGGACTTGGAGC |
| SD | AATCCAGTGGTGGATATGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 137 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15503A-A10 | VGB6 | C57BL/6NTac | |
| 15503A-B2 | VGB6 | C57BL/6NTac | |
| 15503A-D9 | VGB6 | C57BL/6NTac | |
| 15503A-H2 | VGB6 | C57BL/6NTac | |
