15504   Gnpat     Status: ES cell colonies screened / QC positives

GeneGnpat
Chromosome8
Deletion Start124,889,381
Deletion End124,863,241
Deletion Size26,141
Genome Build38
MGI IDMGI:1343460
NCBI Gene ID14712
BAC ID298A16
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 8

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF124,863,414124,863,433AGCGGGTCCTAACCCAGTTG
TUP124,863,436124,863,459TACGTGGCTTGGTATCCACGGCAA
TUR124,863,464124,863,482TTAACCGAGGCGCAGACAC
TDF124,889,071124,889,092CCTAGTGCATGATGCGGGTAAG
TDP124,889,093124,889,118AGCTGCCTGGATGCTATAGGCTCAGA
TDR124,889,162124,889,182TGGTTCCAGTGGGCTCTATTG

PCR Primers

PrimerSequence
SUTGGGAATCAGCACGGAGAC
SDAGTGCCCTGTGGTTTGATTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR69 bp++-
TDFTDR112 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15504A-E6VGB6C57BL/6NTac
15504A-E11VGB6C57BL/6NTac
15504A-H8VGB6C57BL/6NTac
15504A-H3VGB6C57BL/6NTac
15504A-F5VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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