| Gene | Mmp8 |
| Chromosome | 9 |
| Deletion Start | 7,567,549 |
| Deletion End | 7,558,539 |
| Deletion Size | 9,011 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,558,666 | 7,558,686 | TTGATTTGCCGGTGGTTACAG |
| TUP | 7,558,690 | 7,558,716 | TCTGAGGGTGGCTTTCCCTTCTTAGTC |
| TUR | 7,558,731 | 7,558,752 | GCAAACCCATGCGAAGAATCTC |
| TDF | 7,567,214 | 7,567,234 | GGACCCAGGTTACCCCAAAAG |
| TDP | 7,567,238 | 7,567,266 | ACCAAGCATGTTCCCAGGAGTAAACTGTA |
| TDR | 7,567,267 | 7,567,287 | GCAGGAAAACTGCATCGACTC |
PCR Primers
| Primer | Sequence |
| SU | GCAGTGGTGGCTTAGAAACAG |
| SD | CCAACGGAGGAAATAACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15506A-D3 | VGB6 | C57BL/6NTac | |
| 15506A-H12 | VGB6 | C57BL/6NTac | |
