| Gene | Rdh8 |
| Chromosome | 9 |
| Deletion Start | 20,825,754 |
| Deletion End | 20,818,507 |
| Deletion Size | 7,248 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,818,616 | 20,818,639 | CAGCTTCTGGCACTGTCTATTGTC |
| TUP | 20,818,640 | 20,818,665 | ACAGCCTCCTGGGTGCAGGTTGGATA |
| TUR | 20,818,678 | 20,818,698 | TCCTCTGTGTCCAGGGTCTTC |
| TDF | 20,825,166 | 20,825,187 | CCCTCTTCCCAGCATCTCAATG |
| TDP | 20,825,189 | 20,825,208 | TGGAGCCAGGCCCAGTCACC |
| TDR | 20,825,283 | 20,825,305 | GGAGGTACAAGTCCCGGAAGTAG |
PCR Primers
| Primer | Sequence |
| SU | AGCTCAGACTCTCCATCCTC |
| SD | CAATGCAGAACCAAGTGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 140 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15509A-B3 | VGB6 | C57BL/6NTac | |
| 15509A-C2 | VGB6 | C57BL/6NTac | |
| 15509A-D1 | VGB6 | C57BL/6NTac | |
| 15509A-D11 | VGB6 | C57BL/6NTac | |
| 15509A-G12 | VGB6 | C57BL/6NTac | |
