| Gene | Spata19 |
| Chromosome | 9 |
| Deletion Start | 27,400,920 |
| Deletion End | 27,396,872 |
| Deletion Size | 4,049 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,396,988 | 27,397,010 | CCTGTCGTCTCCCTTCTACACTC |
| TUP | 27,397,012 | 27,397,039 | TGCTGTGCTTCAGAGTCATCTCATGTCA |
| TUR | 27,397,045 | 27,397,067 | CACCCTCACCACTAAGACCAATG |
| TDF | 27,400,403 | 27,400,422 | CCAGCCACACCCGTATCTTC |
| TDP | 27,400,423 | 27,400,452 | CAAGTGCCAAGTGAAGTGATGGATGACGTC |
| TDR | 27,400,489 | 27,400,506 | GCGACTCCTCGACAGAAC |
PCR Primers
| Primer | Sequence |
| SU | CATGATGCTGGTCTGAATGG |
| SD | ATCAGTCCTTTCATGCCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15515A-A12 | VGB6 | C57BL/6NTac | |
| 15515A-C1 | VGB6 | C57BL/6NTac | |
| 15515A-E1 | VGB6 | C57BL/6NTac | |
| 15515A-G1 | VGB6 | C57BL/6NTac | |
| 15515A-H12 | VGB6 | C57BL/6NTac | |
| 15515A-E9 | VGB6 | C57BL/6NTac | |
