| Gene | AI118078 |
| Chromosome | 9 |
| Deletion Start | 55,400,642 |
| Deletion End | 55,377,579 |
| Deletion Size | 23,064 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,378,085 | 55,378,105 | ACACGGCTGGGTCACAGAATC |
| TUP | 55,378,109 | 55,378,136 | CAGATGGGTCTGCTGATCTTCTCTGGGA |
| TUR | 55,378,156 | 55,378,175 | GCTGGCAGCTCTACAACAAG |
| TDF | 55,399,987 | 55,400,008 | TGTGGTGTCCTGTCCTTTAGAG |
| TDP | 55,400,013 | 55,400,037 | CTTCCAAGGATGCCTGGCTAGCTGA |
| TDR | 55,400,043 | 55,400,061 | TACTGCGGGCTCCTTACTC |
PCR Primers
| Primer | Sequence |
| SU | GCTGTACCTGCACACAGAGG |
| SD | CTCCTTCTCAGGTTCATCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15525A-B3 | VGB6 | C57BL/6NTac | |
| 15525A-B7 | VGB6 | C57BL/6NTac | |
| 15525A-H10 | VGB6 | C57BL/6NTac | |
| 15525A-G4 | VGB6 | C57BL/6NTac | |
| 15525A-D10 | VGB6 | C57BL/6NTac | |
