| Gene | Echdc1 |
| Chromosome | 10 |
| Deletion Start | 29,334,266 |
| Deletion End | 29,313,670 |
| Deletion Size | 20,597 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,313,717 | 29,313,739 | CCCTTGTGAGAACCGAGGTAATG |
| TUP | 29,313,741 | 29,313,764 | TCCGTGGGATCTCTACCTGTGAGC |
| TUR | 29,313,793 | 29,313,816 | GGCTTCACGAAACAAAGATGTAGG |
| TDF | 29,333,958 | 29,333,979 | GAAGACGGTGAGTCTGCTTTAC |
| TDP | 29,333,982 | 29,334,006 | AAAGCCAGGCAGTGATTCAGGCAGG |
| TDR | 29,334,016 | 29,334,034 | GCTCGTGCTCCAGGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | GAAGAGGGTCTCAGGACACG |
| SD | GAACCCTCTTGATGAGAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15551A-C2 | VGB6 | C57BL/6NTac | |
| 15551A-E1 | VGB6 | C57BL/6NTac | |
| 15551A-E7 | VGB6 | C57BL/6NTac | |
| 15551A-E4 | VGB6 | C57BL/6NTac | |
| 15551A-E2 | VGB6 | C57BL/6NTac | |
