| Gene | Snx3 |
| Chromosome | 10 |
| Deletion Start | 42,534,786 |
| Deletion End | 42,502,334 |
| Deletion Size | 32,453 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,502,915 | 42,502,938 | GACGCTTACCAAGACGTAGTGAAG |
| TUP | 42,502,940 | 42,502,964 | CAAGCTTAGCTGCTCTTTCCAGGCC |
| TUR | 42,502,979 | 42,502,999 | TACGGCCCAGGATACTCTTAC |
| TDF | 42,534,432 | 42,534,453 | AAAGCGCCCTTAACCTGCTGAG |
| TDP | 42,534,458 | 42,534,482 | TTACCACGTTCATGACCCAGAGGGA |
| TDR | 42,534,513 | 42,534,536 | GATGCAAGCATTACTCTGGACAAC |
PCR Primers
| Primer | Sequence |
| SU | ATTGTGGTTATTGTAGTCTCC |
| SD | TGGAGACAAGTGGTGTCGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15552A-B8 | VGB6 | C57BL/6NTac | |
| 15552A-C5 | VGB6 | C57BL/6NTac | |
| 15552A-C11 | VGB6 | C57BL/6NTac | |
| 15552A-E10 | VGB6 | C57BL/6NTac | |
| 15552A-F6 | VGB6 | C57BL/6NTac | |
| 15552A-F11 | VGB6 | C57BL/6NTac | |
