| Gene | Prdm1 |
| Chromosome | 10 |
| Deletion Start | 44,458,525 |
| Deletion End | 44,439,581 |
| Deletion Size | 18,945 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,458,015 | 44,458,034 | CCTTCTCGGAGCGTCCTATC |
| TUP | 44,457,987 | 44,458,013 | AGGGACGAATTGTAAAGACCAGCTCCG |
| TUR | 44,457,960 | 44,457,980 | AAACACAGTACAGCGGGAGTC |
| TDF | 44,440,271 | 44,440,289 | GTCTTCCCTGTGCCCTTTC |
| TDP | 44,440,248 | 44,440,270 | TCTGTCTTCCCTTGCCCTCACAC |
| TDR | 44,440,196 | 44,440,219 | AGGTGGGTCTTGAGATTGCTTGTG |
PCR Primers
| Primer | Sequence |
| SU | GTGTGGGTAAACATGGAGGG |
| SD | ACAAAGAATGGCCACAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15553A-A7 | VGB6 | C57BL/6NTac | |
| 15553A-H9 | VGB6 | C57BL/6NTac | |
| 15553A-B7 | VGB6 | C57BL/6NTac | |
| 15553A-B10 | VGB6 | C57BL/6NTac | |
| 15553A-D8 | VGB6 | C57BL/6NTac | |
| 15553A-A9 | VGB6 | C57BL/6NTac | |
