| Gene | Rfx6 |
| Chromosome | 10 |
| Deletion Start | 51,729,877 |
| Deletion End | 51,711,831 |
| Deletion Size | 18,047 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,712,573 | 51,712,591 | GCAACCTGGGCACTTCATC |
| TUP | 51,712,592 | 51,712,619 | CCCTGTTCAGTAGTCACAGAGCAACAGA |
| TUR | 51,712,671 | 51,712,692 | TCCTTGCTCTCCCTTGTCATTC |
| TDF | 51,729,725 | 51,729,746 | TGCAGCAAGCAACAGAGAAATG |
| TDP | 51,729,748 | 51,729,774 | TGTCCTCTTTACCACCCATCAACACCG |
| TDR | 51,729,833 | 51,729,850 | GGCAGCAGAGCGTTTGAG |
PCR Primers
| Primer | Sequence |
| SU | CAAAATGATGCAAGCTGACATG |
| SD | TTGTAAACATTTCCACAGGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 126 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15555A-D10 | VGB6 | C57BL/6NTac | |
| 15555A-C11 | VGB6 | C57BL/6NTac | |
| 15555A-E9 | VGB6 | C57BL/6NTac | |
| 15555A-D4 | VGB6 | C57BL/6NTac | |
