| Gene | Adora2a |
| Chromosome | 10 |
| Deletion Start | 75,333,984 |
| Deletion End | 75,326,032 |
| Deletion Size | 7,953 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,326,263 | 75,326,282 | TGCTTTGTCCTGGTCCTCAC |
| TUP | 75,326,289 | 75,326,316 | TTCCATCTTCAGCCTCTTGGCTATTGCC |
| TUR | 75,326,320 | 75,326,340 | TCGGATGGCGATGTATCTGTC |
| TDF | 75,333,034 | 75,333,053 | GGCTTGGTGACGGGTATGAG |
| TDP | 75,333,056 | 75,333,081 | CGAAGGGCATCATTGCGATTTGCTGG |
| TDR | 75,333,087 | 75,333,105 | CAGGCCGATGGCGAATGAC |
PCR Primers
| Primer | Sequence |
| SU | AGGGTGGTTCAGCTCTTCAC |
| SD | GCCACTTCTAGGTCAGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15561A-B1 | VGB6 | C57BL/6NTac | |
| 15561A-H8 | VGB6 | C57BL/6NTac | |
| 15561A-F6 | VGB6 | C57BL/6NTac | |
| 15561A-E12 | VGB6 | C57BL/6NTac | |
| 15561A-E3 | VGB6 | C57BL/6NTac | |
| 15561A-B5 | VGB6 | C57BL/6NTac | |
