| Gene | Susd2 |
| Chromosome | 10 |
| Deletion Start | 75,643,940 |
| Deletion End | 75,637,280 |
| Deletion Size | 6,661 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,643,523 | 75,643,541 | CTCGGCGCAACCAAGAAAG |
| TUP | 75,643,495 | 75,643,521 | CTAGCAGGAAGGATGAGCCTAGAGCCA |
| TUR | 75,643,471 | 75,643,490 | CCAGCTCAGCTCAGGGAAAG |
| TDF | 75,637,804 | 75,637,825 | ACGGGCATAAGGAAGGCTTGAG |
| TDP | 75,637,775 | 75,637,800 | CCTGGAAGGATCCGTTGTCCGATTCA |
| TDR | 75,637,723 | 75,637,740 | GCCTGACAGGTGCTACTC |
PCR Primers
| Primer | Sequence |
| SU | CAGGTGCCAAAGGTCTTAGC |
| SD | CCGAGCTCTCAAGTTGTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15562A-B9 | VGB6 | C57BL/6NTac | |
| 15562A-B12 | VGB6 | C57BL/6NTac | |
| 15562A-H11 | VGB6 | C57BL/6NTac | |
| 15562A-D12 | VGB6 | C57BL/6NTac | |
| 15562A-C10 | VGB6 | C57BL/6NTac | |
| 15562A-C9 | VGB6 | C57BL/6NTac | |
