| Gene | Pcnt |
| Chromosome | 10 |
| Deletion Start | 76,442,615 |
| Deletion End | 76,418,364 |
| Deletion Size | 24,252 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,442,355 | 76,442,373 | TCGCTCCGCCCTATTACAC |
| TUP | 76,442,330 | 76,442,354 | CCACAGCCAATCAGCGTCAGAGACG |
| TUR | 76,442,254 | 76,442,275 | CACCGGAAGCACAACAACTAAG |
| TDF | 76,418,591 | 76,418,611 | AGGATATGCACAGGCTTGACC |
| TDP | 76,418,565 | 76,418,589 | CTGTGGGAACTCTGTCTCAGCAGCA |
| TDR | 76,418,525 | 76,418,543 | CAGCAGGTGCCCTTCATTG |
PCR Primers
| Primer | Sequence |
| SU | GTGTTCGCAGAAACACATCG |
| SD | GGACGAGTGAGGGAAAACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15563A-B4 | VGB6 | C57BL/6NTac | |
| 15563A-E8 | VGB6 | C57BL/6NTac | |
| 15563A-A8 | VGB6 | C57BL/6NTac | |
| 15563A-E10 | VGB6 | C57BL/6NTac | |
