| Gene | Col18a1 |
| Chromosome | 10 |
| Deletion Start | 77,096,533 |
| Deletion End | 77,067,383 |
| Deletion Size | 29,151 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,095,726 | 77,095,746 | CCAGGCTCCCATTCTGAGTTG |
| TUP | 77,095,700 | 77,095,724 | TCCAGACCACCTTCTCAGGCATGAA |
| TUR | 77,095,670 | 77,095,689 | GTTTGGTGCCTCCAGCTTTC |
| TDF | 77,067,777 | 77,067,797 | TGCTAGTGTCCACCTGTCCTG |
| TDP | 77,067,751 | 77,067,775 | TGGCATGGTTATCCGCCTTGGTTGT |
| TDR | 77,067,705 | 77,067,723 | CCGACTGCCAGAGTCCTTG |
PCR Primers
| Primer | Sequence |
| SU | TGATGTATCACCTAGCTGGC |
| SD | TCATGAAGTCACAGTCCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15565A-A11 | VGB6 | C57BL/6NTac | |
| 15565A-H11 | VGB6 | C57BL/6NTac | |
| 15565A-F8 | VGB6 | C57BL/6NTac | |
