| Gene | Casp14 |
| Chromosome | 10 |
| Deletion Start | 78,715,855 |
| Deletion End | 78,713,318 |
| Deletion Size | 2,538 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,715,333 | 78,715,352 | GGGAGGGTTCCGAGGTAGAC |
| TUP | 78,715,309 | 78,715,331 | TGGAGGCCCTGGAACGCATGTTC |
| TUR | 78,715,236 | 78,715,255 | TGACATGGAGCTGGCTGTTC |
| TDF | 78,714,241 | 78,714,262 | ACTCCACGGTAGAGGGTATGAG |
| TDP | 78,714,210 | 78,714,236 | CAGATGTCCTAGGGTGCCTTATGCTCC |
| TDR | 78,714,180 | 78,714,201 | CAGGGATCTTGGGTGCTGATTC |
PCR Primers
| Primer | Sequence |
| SU | CCAAACCCAAGCAAGTAAATG |
| SD | CACAGTGGCGATCTTAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15566B-A11 | VGB6 | C57BL/6NTac | |
| 15566B-E7 | VGB6 | C57BL/6NTac | |
| 15566B-H10 | VGB6 | C57BL/6NTac | |
| 15566B-H5 | VGB6 | C57BL/6NTac | |
| 15566C-C1 | VGB6 | C57BL/6NTac | |
