| Gene | Dram1 |
| Chromosome | 10 |
| Deletion Start | 88,356,919 |
| Deletion End | 88,324,693 |
| Deletion Size | 32,227 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,356,110 | 88,356,130 | GGATGGACCTGCCTCATACTC |
| TUP | 88,356,084 | 88,356,108 | TCTGGACCTGGTGCCTCTAAAGTCA |
| TUR | 88,356,000 | 88,356,022 | CCTCAGGAAAGACGGAGACTAAG |
| TDF | 88,324,878 | 88,324,897 | AAGGCACGGTAGTGGGAGTC |
| TDP | 88,324,838 | 88,324,867 | TGGGAGAAAGCGTCTGAGAATGGCATACTT |
| TDR | 88,324,788 | 88,324,805 | ACAGAGGCAGTGGGAAGG |
PCR Primers
| Primer | Sequence |
| SU | TGGAGAGAGAGGCTCAGAGG |
| SD | TGAATATGCCCTGATTTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 131 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15572A-H12 | VGB6 | C57BL/6NTac | |
| 15572A-B10 | VGB6 | C57BL/6NTac | |
| 15572A-H5 | VGB6 | C57BL/6NTac | |
