| Gene | Kcnc2 |
| Chromosome | 10 |
| Deletion Start | 112,272,400 |
| Deletion End | 112,271,709 |
| Deletion Size | 692 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,271,769 | 112,271,786 | TACCGCAGCACCCTCAAG |
| TUP | 112,271,787 | 112,271,808 | ACCCTGCCTGGAACTCGCCTGG |
| TUR | 112,271,811 | 112,271,832 | GAGGTTCAGAGGAGGCAAGAAG |
| TDF | 112,272,062 | 112,272,083 | CAATTACTACCGCACGGGCAAG |
| TDP | 112,272,100 | 112,272,119 | ACGTGTGCGGGCCGCTCTTC |
| TDR | 112,272,147 | 112,272,165 | GCTCCACGTCGGTCTCATC |
PCR Primers
| Primer | Sequence |
| SU | AGGTTTCCTGGAAGGTCAAG |
| SD | AGGACACATCCCAGGACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15577A-A1 | VGB6 | C57BL/6NTac | |
| 15577A-A3 | VGB6 | C57BL/6NTac | |
| 15577A-F7 | VGB6 | C57BL/6NTac | |
