| Gene | Helb |
| Chromosome | 10 |
| Deletion Start | 120,112,859 |
| Deletion End | 120,084,671 |
| Deletion Size | 28,189 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,112,583 | 120,112,603 | GACTGTTGACAGACAGACCTG |
| TUP | 120,112,557 | 120,112,579 | TGTGACTGGCTGCTTCACCGTAC |
| TUR | 120,112,517 | 120,112,538 | ATGTGCTCTGAGTCATTACGAC |
| TDF | 120,084,813 | 120,084,836 | CAGCAATCAGGAGATGGAAATGTG |
| TDP | 120,084,790 | 120,084,812 | AGGATCTGCGTTCATGGCCAGCC |
| TDR | 120,084,760 | 120,084,778 | GGGCTGAGCAGGGTCTTAC |
PCR Primers
| Primer | Sequence |
| SU | TTCACCACGTCTCCTTCTCC |
| SD | TAAGCATACAGCACCTTTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15578A-D4 | VGB6 | C57BL/6NTac | |
| 15578A-B4 | VGB6 | C57BL/6NTac | |
| 15578A-C12 | VGB6 | C57BL/6NTac | |
| 15578A-E12 | VGB6 | C57BL/6NTac | |
| 15578A-A5 | VGB6 | C57BL/6NTac | |
| 15578A-C11 | VGB6 | C57BL/6NTac | |
