| Gene | B4galnt1 |
| Chromosome | 10 |
| Deletion Start | 127,171,839 |
| Deletion End | 127,166,008 |
| Deletion Size | 5,832 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,166,761 | 127,166,781 | AGCCTTCCCTTGCCCTTTCTG |
| TUP | 127,166,785 | 127,166,808 | CAGGTTCGGGCGGTTGACCTCACT |
| TUR | 127,166,822 | 127,166,842 | AAACAGCCCTCAGCTCCTCAG |
| TDF | 127,171,750 | 127,171,769 | CCGGGATCACTGGACCAAAG |
| TDP | 127,171,770 | 127,171,794 | TCAAGTGGCCAAACATCGCCTACTC |
| TDR | 127,171,810 | 127,171,828 | CGGCGGTCATGCACTGTAG |
PCR Primers
| Primer | Sequence |
| SU | CCAGGTGCAGTACAGCCG |
| SD | TCGGGAGGTGAGAGTGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15582A-B12 | VGB6 | C57BL/6NTac | |
| 15582A-E3 | VGB6 | C57BL/6NTac | |
| 15582A-F11 | VGB6 | C57BL/6NTac | |
| 15582A-G8 | VGB6 | C57BL/6NTac | |
