| Gene | Gli1 |
| Chromosome | 10 |
| Deletion Start | 127,338,517 |
| Deletion End | 127,330,034 |
| Deletion Size | 8,484 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,338,413 | 127,338,435 | GCATGGGAACAGAAGGTGAATAC |
| TUP | 127,338,387 | 127,338,411 | TTTACCAGTCCCTGGGCCTTGAGGA |
| TUR | 127,338,332 | 127,338,353 | ATGCGCTAGGGATGGAGTTGAG |
| TDF | 127,330,893 | 127,330,912 | GGTCTGACTCCACCGGATTG |
| TDP | 127,330,870 | 127,330,891 | CACCCTGCCTCAATGCCCACCC |
| TDR | 127,330,779 | 127,330,799 | CTGAGGATAGGGACCCGACTG |
PCR Primers
| Primer | Sequence |
| SU | CCCACTCTTTGGGATGTTTC |
| SD | TGATGCTCAACAACTTTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 134 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15583A-B7 | VGB6 | C57BL/6NTac | |
| 15583A-H2 | VGB6 | C57BL/6NTac | |
| 15583A-C2 | VGB6 | C57BL/6NTac | |
| 15583A-D4 | VGB6 | C57BL/6NTac | |
| 15583A-F4 | VGB6 | C57BL/6NTac | |
| 15583A-E1 | VGB6 | C57BL/6NTac | |
