| Gene | Sdr9c7 |
| Chromosome | 10 |
| Deletion Start | 127,910,065 |
| Deletion End | 127,898,648 |
| Deletion Size | 11,418 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,898,862 | 127,898,882 | TGCAGACCTTCCTCCTAGATG |
| TUP | 127,898,883 | 127,898,907 | TCACCAAGTCGGAGAACGTCAAGGA |
| TUR | 127,898,915 | 127,898,933 | CTTGGTCCCTCACCCACTG |
| TDF | 127,909,849 | 127,909,866 | TACAACCCTGGCCTGGAC |
| TDP | 127,909,868 | 127,909,897 | TCAAGTTTCTCTATCTCACCCTGGCTAAGC |
| TDR | 127,909,931 | 127,909,950 | TGCTGGTCTTGGCAAGTATC |
PCR Primers
| Primer | Sequence |
| SU | TGACAAGCTTAAATGCACGG |
| SD | AAACCAGTGCCTGCTTTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15585A-G7 | VGB6 | C57BL/6NTac | |
| 15585A-F5 | VGB6 | C57BL/6NTac | |
| 15585A-H6 | VGB6 | C57BL/6NTac | |
| 15585A-H8 | VGB6 | C57BL/6NTac | |
| 15585A-E11 | VGB6 | C57BL/6NTac | |
