| Gene | Itga7 |
| Chromosome | 10 |
| Deletion Start | 128,957,943 |
| Deletion End | 128,933,992 |
| Deletion Size | 23,952 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 128,934,416 | 128,934,439 | CTCTCCCTTCCGTTTGCATTCTTG |
| TUP | 128,934,442 | 128,934,463 | CCAGCCTTGCATCTCTGGCTCC |
| TUR | 128,934,481 | 128,934,500 | CCCTTCCTGGAGCAGTTCAC |
| TDF | 128,957,837 | 128,957,855 | CCAGCCACTGCCTAACATC |
| TDP | 128,957,858 | 128,957,877 | CTGTCCCAAGCCCAGCCCGT |
| TDR | 128,957,910 | 128,957,932 | TACTCCACTATCTCCATCCCAAG |
PCR Primers
| Primer | Sequence |
| SU | GGTGGGTGAAGGAATGAGTG |
| SD | AGAGCGGCACTCAATAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15587A-H12 | VGB6 | C57BL/6NTac | |
| 15587A-D6 | VGB6 | C57BL/6NTac | |
| 15587A-G3 | VGB6 | C57BL/6NTac | |
| 15587A-C10 | VGB6 | C57BL/6NTac | |
| 15587A-H4 | VGB6 | C57BL/6NTac | |
| 15587A-F2 | VGB6 | C57BL/6NTac | |
