| Gene | Neurod4 |
| Chromosome | 10 |
| Deletion Start | 130,271,400 |
| Deletion End | 130,270,319 |
| Deletion Size | 1,082 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,270,996 | 130,271,016 | CGACTGGCAAGGAACTACATC |
| TUP | 130,270,973 | 130,270,995 | TGGGCCTTGTCTGAAGTCCTGGA |
| TUR | 130,270,941 | 130,270,963 | CAAATCCCTTCCCTTCAAGTGTC |
| TDF | 130,270,545 | 130,270,569 | ATGCCACATTATACCTCTGCAAGTC |
| TDP | 130,270,516 | 130,270,539 | TCAGGGCATGTGCATTCAACTCCC |
| TDR | 130,270,441 | 130,270,462 | TCCAATGCTATGGTGGGAGTAG |
PCR Primers
| Primer | Sequence |
| SU | GCAATGTGCTGTAAGTAGATG |
| SD | AATGTGTGGGAGGTGAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 129 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15588A-E11 | VGB6 | C57BL/6NTac | |
| 15588A-F5 | VGB6 | C57BL/6NTac | |
