| Gene | Ddx56 |
| Chromosome | 11 |
| Deletion Start | 6,267,716 |
| Deletion End | 6,259,166 |
| Deletion Size | 8,551 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,267,565 | 6,267,588 | CCTTCTTAGGCTGTCACCGATTTG |
| TUP | 6,267,536 | 6,267,558 | TCGCGACCTACGTTGATCCAGGA |
| TUR | 6,267,512 | 6,267,529 | TCCAGCGCCAGAGGAATG |
| TDF | 6,259,624 | 6,259,642 | TGAGGCCCAGCATCTCTTC |
| TDP | 6,259,599 | 6,259,620 | TTCCTGCTGCTCTTCGTGGCCT |
| TDR | 6,259,566 | 6,259,587 | GGCACCTTTCTCCGTTTCTTTC |
PCR Primers
| Primer | Sequence |
| SU | TCTGGTTACCCACAGAACCC |
| SD | GCAGGAACTACAGACAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15591A-E3 | VGB6 | C57BL/6NTac | |
| 15591A-B5 | VGB6 | C57BL/6NTac | |
