| Gene | Aftph |
| Chromosome | 11 |
| Deletion Start | 20,727,604 |
| Deletion End | 20,712,168 |
| Deletion Size | 15,437 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,727,001 | 20,727,023 | GGGAACAGATGACCTGGACAATG |
| TUP | 20,726,976 | 20,727,000 | TGGCAGATTCAAAGGGATCGAAGCC |
| TUR | 20,726,947 | 20,726,967 | AAATGCACTCGGTGCCACAAG |
| TDF | 20,713,145 | 20,713,167 | GGATGGCTTTGTGGGTAGAAGTG |
| TDP | 20,713,117 | 20,713,139 | TGCCAAGCCAGAAGAGCTCAGGT |
| TDR | 20,713,083 | 20,713,102 | AGGCCGACTGTTGGGAGATG |
PCR Primers
| Primer | Sequence |
| SU | AGGTGGTTCCCTAAAGCCTG |
| SD | AGCAGGTCTACACTGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15593C-A8 | VGB6 | C57BL/6NTac | |
| 15593C-H12 | VGB6 | C57BL/6NTac | |
| 15593B-A6 | VGB6 | C57BL/6NTac | |
| 15593B-B9 | VGB6 | C57BL/6NTac | |
| 15593B-G10 | VGB6 | C57BL/6NTac | |
