| Gene | Otx1 |
| Chromosome | 11 |
| Deletion Start | 21,999,473 |
| Deletion End | 21,996,236 |
| Deletion Size | 3,238 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,998,826 | 21,998,847 | GTCCTGTTTCTGTCCACCTCTG |
| TUP | 21,998,793 | 21,998,815 | TGCCTCTCAGTTAGGGAGCGTGT |
| TUR | 21,998,762 | 21,998,781 | GCCACCCTTTCCTTCTCATC |
| TDF | 21,996,639 | 21,996,657 | CGCAGCCTCCTACCCTATG |
| TDP | 21,996,616 | 21,996,637 | CCTATGGCCAGGGCGGAAGCTA |
| TDR | 21,996,564 | 21,996,582 | GCAGTCTACACCGCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GAGTGTCTTTGGCCCTTGTC |
| SD | CAGAGGGTCAGAGCGAAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15594A-A10 | VGB6 | C57BL/6NTac | |
| 15594B-C10 | VGB6 | C57BL/6NTac | |
| 15594C-A3 | VGB6 | C57BL/6NTac | |
| 15594C-G8 | VGB6 | C57BL/6NTac | |
| 15594C-D1 | VGB6 | C57BL/6NTac | |
