| Gene | Srebf1 |
| Chromosome | 11 |
| Deletion Start | 60,220,540 |
| Deletion End | 60,199,903 |
| Deletion Size | 20,638 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,220,265 | 60,220,285 | TCAACTTCAAGCCTGGTCCTG |
| TUP | 60,220,242 | 60,220,264 | TGTACAGTTCACAGGCCGACCCG |
| TUR | 60,220,182 | 60,220,205 | GCAAATATCGGTTCGTGGACTTTC |
| TDF | 60,200,752 | 60,200,772 | CAGTCACCAGCTTCAGTCCAG |
| TDP | 60,200,726 | 60,200,749 | AGCTCACGGTACCAGCAATGGACC |
| TDR | 60,200,676 | 60,200,694 | GGCTGCTCAGGTCATGTTG |
PCR Primers
| Primer | Sequence |
| SU | ATCTAACGATGTCTGGGCTG |
| SD | AGCAATCAGCTGAGGCAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15601B-H4 | VGB6 | C57BL/6NTac | |
| 15601B-B4 | VGB6 | C57BL/6NTac | |
| 15601B-D4 | VGB6 | C57BL/6NTac | |
| 15601B-E3 | VGB6 | C57BL/6NTac | |
| 15601B-H2 | VGB6 | C57BL/6NTac | |
| 15601B-G4 | VGB6 | C57BL/6NTac | |
