| Gene | Adora2b |
| Chromosome | 11 |
| Deletion Start | 62,265,770 |
| Deletion End | 62,249,105 |
| Deletion Size | 16,666 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,249,575 | 62,249,592 | TCTCCAGCACGCTTGTTC |
| TUP | 62,249,593 | 62,249,616 | TGCCGGCTATCAAGACTCTCCTGG |
| TUR | 62,249,624 | 62,249,641 | CCAGTCAGTGGCCTGTTC |
| TDF | 62,265,085 | 62,265,105 | CACGAGCGAGAGGGATCATTG |
| TDP | 62,265,109 | 62,265,131 | TCCTCTGGGTCCTTGCCTTTGGC |
| TDR | 62,265,161 | 62,265,180 | TGGTGGCACTGTCTTTACTG |
PCR Primers
| Primer | Sequence |
| SU | GTGAAGTCTGGGACTTCGC |
| SD | AAAGCTTAGGATAGAATCATTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15602A-A5 | VGB6 | C57BL/6NTac | |
| 15602A-G8 | VGB6 | C57BL/6NTac | |
| 15602A-F5 | VGB6 | C57BL/6NTac | |
