| Gene | Gucy2e |
| Chromosome | 11 |
| Deletion Start | 69,236,642 |
| Deletion End | 69,223,017 |
| Deletion Size | 13,626 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,235,860 | 69,235,878 | TCCTGCCGAGAGTGGACAG |
| TUP | 69,235,836 | 69,235,857 | TAGCGAGCCAGCCTCTGTGTCC |
| TUR | 69,235,792 | 69,235,811 | AGCAGCACCGAGTGCATCAC |
| TDF | 69,223,345 | 69,223,367 | CTTCAACAAGCCCATTCCCAAAC |
| TDP | 69,223,321 | 69,223,344 | CACCTGATCTGCAGCCAGGGTGAG |
| TDR | 69,223,282 | 69,223,301 | CGCCAGCATCCAGCTTTCTC |
PCR Primers
| Primer | Sequence |
| SU | CTTCGGTCCTGTATGAAGGC |
| SD | CTGACAGCCTATGAGCTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15604A-E1 | VGB6 | C57BL/6NTac | |
