| Gene | Senp3 |
| Chromosome | 11 |
| Deletion Start | 69,680,836 |
| Deletion End | 69,673,482 |
| Deletion Size | 7,355 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,680,693 | 69,680,712 | GTGGTGGTTTTGGGCCAGAT |
| TUP | 69,680,671 | 69,680,692 | CCTGGGTCTGGGACCACAGTGC |
| TUR | 69,680,652 | 69,680,670 | GCAGGGAGGCGTCTAGTTG |
| TDF | 69,674,368 | 69,674,389 | CTTGGAGTCCGAGGAGTGAGTG |
| TDP | 69,674,341 | 69,674,365 | TGTGATGTTTCCCACCAGCACACTC |
| TDR | 69,674,313 | 69,674,337 | CACCTTAATCACCTGCCAGTGTAAC |
PCR Primers
| Primer | Sequence |
| SU | AGCATTAAAGCTGAGCCTGG |
| SD | TATTGACCAGAGCAGGACCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 61 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15605A-G8 | VGB6 | C57BL/6NTac | |
| 15605A-C6 | VGB6 | C57BL/6NTac | |
| 15605A-H8 | VGB6 | C57BL/6NTac | |
