| Gene | Asgr1 |
| Chromosome | 11 |
| Deletion Start | 70,057,706 |
| Deletion End | 70,054,903 |
| Deletion Size | 2,804 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,055,660 | 70,055,680 | GCACAGGATTCACGGAAAGAG |
| TUP | 70,055,682 | 70,055,708 | AGGAAACCCACCCTTCCCACACTTTGG |
| TUR | 70,055,730 | 70,055,752 | TGTCATTACAGACGATGAACCAC |
| TDF | 70,057,170 | 70,057,188 | CCAGGGATGAGCAGGTGAG |
| TDP | 70,057,190 | 70,057,213 | CCACGCTGAGCTGGGTTCTTCTGG |
| TDR | 70,057,259 | 70,057,278 | GCGCTGGAGGAAGTTCTGAG |
PCR Primers
| Primer | Sequence |
| SU | GACAGCCAAGAGAGAGTGGC |
| SD | CCTCCCATCTTCCAACTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15606A-C4 | VGB6 | C57BL/6NTac | |
| 15606A-G3 | VGB6 | C57BL/6NTac | |
| 15606A-C10 | VGB6 | C57BL/6NTac | |
| 15606A-E3 | VGB6 | C57BL/6NTac | |
| 15606A-A1 | VGB6 | C57BL/6NTac | |
