| Gene | Prpf8 |
| Chromosome | 11 |
| Deletion Start | 75,509,354 |
| Deletion End | 75,487,095 |
| Deletion Size | 22,260 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,487,935 | 75,487,957 | GCCCTGGATTTAGCTCTGTAGAC |
| TUP | 75,487,958 | 75,487,981 | CGGGCTGCCCTTTAACCGATAGGG |
| TUR | 75,488,001 | 75,488,021 | GCCTTCAATCCCAGCTCTTTG |
| TDF | 75,509,020 | 75,509,044 | CATGCTTGGTCATCTTGTCATCTTG |
| TDP | 75,509,048 | 75,509,070 | TCTGGCTGCCTTGGAAACGGTCC |
| TDR | 75,509,086 | 75,509,104 | AAGGTTCTGCCTGCCTCAC |
PCR Primers
| Primer | Sequence |
| SU | ATCTCGGCGTCTATTGGATG |
| SD | GGAGGACCACACAGTCCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15616A-F8 | VGB6 | C57BL/6NTac | |
| 15616A-C9 | VGB6 | C57BL/6NTac | |
| 15616B-C1 | VGB6 | C57BL/6NTac | |
| 15616B-E3 | VGB6 | C57BL/6NTac | |
