| Gene | Abcc3 |
| Chromosome | 11 |
| Deletion Start | 94,392,912 |
| Deletion End | 94,363,447 |
| Deletion Size | 29,466 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,392,866 | 94,392,886 | GGGCTCCAAGTTCTGGGTAAG |
| TUP | 94,392,839 | 94,392,865 | AAGCGTCTCTTGGTATCCGTGCTACGG |
| TUR | 94,392,808 | 94,392,827 | CCAGACATCCCACGGTTGAC |
| TDF | 94,364,205 | 94,364,224 | TCCTCATGCTCCTCCCTCTG |
| TDP | 94,364,181 | 94,364,203 | CGGTGCTCCAGGGATCCTCTCCT |
| TDR | 94,364,140 | 94,364,163 | GGGTCTAGGACATTGGAAGAAGTG |
PCR Primers
| Primer | Sequence |
| SU | TCATTCTCACGTCCATCAGC |
| SD | CCCTTCTACTACCCTGCATA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15629A-D2 | VGB6 | C57BL/6NTac | |
| 15629A-D4 | VGB6 | C57BL/6NTac | |
| 15629A-D8 | VGB6 | C57BL/6NTac | |
