| Gene | Acsf2 |
| Chromosome | 11 |
| Deletion Start | 94,601,767 |
| Deletion End | 94,572,230 |
| Deletion Size | 29,538 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,600,874 | 94,600,892 | TTGGCTGTGCAACAAGGTG |
| TUP | 94,600,846 | 94,600,871 | TGGTGGTATCCTCTGCGTCCAGTAGG |
| TUR | 94,600,815 | 94,600,834 | GTGCGGTAGAGCAGGATAGG |
| TDF | 94,572,668 | 94,572,689 | GTTGTCACCTCACCTGTTGTTC |
| TDP | 94,572,642 | 94,572,665 | TTCTGCTTGCACCAGGTGGACAAA |
| TDR | 94,572,610 | 94,572,629 | CGGAGGCCAATGCTCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | TGTATTGTGCTGCAGAGCCG |
| SD | ACCCTGATATACATACTCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15630B-B12 | VGB6 | C57BL/6NTac | |
| 15630B-C4 | VGB6 | C57BL/6NTac | |
| 15630B-D4 | VGB6 | C57BL/6NTac | |
| 15630B-F3 | VGB6 | C57BL/6NTac | |
