| Gene | Ppp1r9b |
| Chromosome | 11 |
| Deletion Start | 95,005,373 |
| Deletion End | 94,991,551 |
| Deletion Size | 13,823 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,992,442 | 94,992,463 | CCGGTGGAGGTTGAAGAAAGTG |
| TUP | 94,992,467 | 94,992,488 | AGTCTGAGGCCGAGTCGGCTCC |
| TUR | 94,992,528 | 94,992,547 | GGGTGCTGCCATTCTCCAAG |
| TDF | 95,005,127 | 95,005,144 | TGGGCAGCCTACAGAGTG |
| TDP | 95,005,145 | 95,005,171 | AGCAGCATGGAAGCTGGAATCCAGTAC |
| TDR | 95,005,188 | 95,005,208 | GCACCACAATCCTTCCCAAAC |
PCR Primers
| Primer | Sequence |
| SU | GCTTCTTCAATTTGGGTGGG |
| SD | AGCCTCCAAGTGGTGAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15631A-A2 | VGB6 | C57BL/6NTac | |
| 15631A-B8 | VGB6 | C57BL/6NTac | |
| 15631A-D5 | VGB6 | C57BL/6NTac | |
| 15631A-D6 | VGB6 | C57BL/6NTac | |
| 15631A-H2 | VGB6 | C57BL/6NTac | |
