15633   Arl5c     Status: ES cell colonies screened / QC positives

GeneArl5c
Chromosome11
Deletion Start97,995,860
Deletion End97,990,224
Deletion Size5,637
Genome Build38
MGI IDMGI:3028577
NCBI Gene ID217151
BAC ID256B13
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,995,11797,995,136TGCCTCCAGAGCACAAAGTC
TUP97,995,08897,995,116ATTATCGTGGGACTGGACAATGCAGGGAA
TUR97,995,03097,995,048AGGCCCAGCCCACTCTATC
TDF97,990,28997,990,307AGGCCACCGCCAACTGATG
TDP97,990,26997,990,288TCCTAGCCCGAGGCCAACCA
TDR97,990,24597,990,268CACTGGTGCTAGTCATTTGGTTTC

PCR Primers

PrimerSequence
SUTGCTCACTCTACTCGCAACAC
SDTCATCAACTCTCTGCCTCCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR107 bp++-
TDFTDR63 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15633A-E10VGB6C57BL/6NTac
15633A-A12VGB6C57BL/6NTac
15633A-H2VGB6C57BL/6NTac
15633A-B2VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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