| Gene | Cdk12 |
| Chromosome | 11 |
| Deletion Start | 98,231,080 |
| Deletion End | 98,203,371 |
| Deletion Size | 27,710 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,203,572 | 98,203,592 | CCACTGGTGGAGTACGATGAC |
| TUP | 98,203,594 | 98,203,621 | TCAGCTCTGATTCAGACACCTTCTCCGA |
| TUR | 98,203,634 | 98,203,654 | TTCTCCCTCCGGTCTGATTTG |
| TDF | 98,230,399 | 98,230,423 | TTGTACTAGCTGTCCTAGAATTGAC |
| TDP | 98,230,425 | 98,230,448 | CTGTAAGCCAGGCTGGCATTGACC |
| TDR | 98,230,455 | 98,230,475 | CAGGCAAAGGCAGGTGTACTG |
PCR Primers
| Primer | Sequence |
| SU | CCTTCCTACTTCCTTGAGCG |
| SD | GGGAGACCTGAGATAAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15634A-E5 | VGB6 | C57BL/6NTac | |
| 15634A-F2 | VGB6 | C57BL/6NTac | |
